Second letter written before, essentially describing a line that’s been crossed (7)

Guardian Cryptic #29031 | 1 Down | 2023-03-30
Generating...

I haven't cracked this one yet — but the brilliant team at Fifteensquared have:

definition: a hybrid / ‘cross’ between cows and bison/buffalo BEE (B, the “Second letter” in the alphabet) + FO (the centre/essential part of be-FO-re) around A + L (line)

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